There are several reasons why the "Bead Count" column in the "Quality Control" tab could be low or zero:
- Astrolabe currently only identifies mass cytometry (CyTOF) beads. If this is a flow cytometry experiment, no beads will be found.
- The Astrolabe debarcoding module removes the beads. In this case, check out the "Debarcoding Report" export for bead quality control statistics.
- If you debarcoded the data yourself, the bead would have been removed as part of the process. There is no faithful way to keep the beads when debarcoding. Make sure to QC them ahead of debarcoding.
- If you used the Fluidigm bead normalization function in the CyTOF software, it is possible the beads were removed. We strongly recommend not removing the beads in this case as they are a valuable quality control instrument.
In each of these cases, the "Oxidation Ratio" and "Signal Stability" columns will either say "No data" or can be safely ignored.
If you're concerned that the Astrolabe analysis did not identify or remove the beads correctly, please contact us at email@example.com. Make sure to include your experiment ID, which can be found under the "Experiment Details" button in the top right.